Lab protocols

Below are links to commonly used Larracuente Lab protocols.

Slot blot showing copy number variation of  repeats in Drosophila melanogaster. Credit: D. Eickbush

Squish prep

“Squish +” prep

DNA slot blots

Total RNA isolation

Chemiluminescent hybridization and detection

FISH showing the hybridization of a 1.688 satellite DNA probe on D. melanogaster mitotic chromosomes.

FISH on squashed tissue


Computational protocols

Please visit our GitHub page for computational pipelines: