Lab protocols

Below are links to commonly used Larracuente Lab protocols.

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Slot blot showing copy number variation of  repeats in Drosophila melanogaster. Credit: D. Eickbush

Squish prep

“Squish +” prep

DNA slot blots

Total RNA isolation

Chemiluminescent hybridization and detection

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FISH showing the hybridization of a 1.688 satellite DNA probe on D. melanogaster mitotic chromosomes.

FISH on squashed tissue

 

Computational protocols

Please visit our GitHub page for computational pipelines: https://github.com/LarracuenteLab/.