Cell Media (EMT6)
- 500 mL BME (Basal Medium Eagle. We have always gotten this from the Foster lab)
- 2.5 mL Pen Strep
- 2 mL Fungizone
- 1 mL plasmocin
- 1 mL primocin
- 10% FBS – 55.5 mL (Fetal Bovine Serum)
*Note: 20% FBS media is also needed for when cells are first thawed.
*For SCC7 cell line, components are all the same except instead of BME base media, use RPMI media
Cell Media (HeLa)
- 500 mL RPMI 1640
- 10% FBS (50 mL)
- 5 mL Hepes Buffer
- 5 mL Pen Strep
*All Nada solutions are based on 500mL total final solution
Nada Solution #1 (basic cell measurement solution)
- 3.9447 g NaCl
- 0.2199 g KCl
- 0.9008 g D-glucose
- 6 mL Hepes
- 0.0832 g CaCl2
- 0.0571 g MgCl2
Nada Solution #2 (permeabilizing solution)
- 3.9447 g NaCl
- 0.2199 g KCl
- 0.9008 g D-glucose
- 6 mL Hepes
- 0.476 g MgCl2
- 20 μM Ionomycin
Nada Solution #3 (calcium shock solution)
- 3.9447g NaCl
- 0.2199g KCl
- 0.9008g D-glucose
- 6mL Hepes
- 0.0888g CaCl2
SUPERFASLIGAND (for 100 ng/10 uL concentration)
- 5 ug of SFL
- 500 uL of HeLa Media