Who: Dan Christensen
What: Application of Random Access Multiphoton (RAMP) Microscopy to Capillary-level Cerebral Blood Flow Imaging in Mice In Vivo
Date: 08/01/2013
Time: 11:30 am – 12:30 pm
Where: Sloan Auditorium (Goergen 101)
Abstract: It’s a bird, it’s a plane, no…it’s a mouse with half of its skull removed strapped to a translation stage! Come hear about how we’re measuring capillary-level blood flow in the brain and the microscope we developed to do it. It’s a familiar tale of joy intermixed with heartache, triumph laced with struggle, and long days accompanied by short nights. This gripping presentation will introduce you to the messy world of live-animal imaging and the optics and electronics we use to do it. Specifically, we have constructed a laser-scanning two-photon microscope employing acousto-optical deflectors (AODs) rather than traditional mirror galvanometers. This gives our microscope a temporal advantage over commercially available systems, allowing us to track individual red blood cells throughout multiple capillaries inside the brain simultaneously.
Bring your lunch! Snacks and beverages will be provided.
