{"id":4192,"date":"2016-02-17T19:54:36","date_gmt":"2016-02-17T19:54:36","guid":{"rendered":"http:\/\/blogs.rochester.edu\/larracuente\/?page_id=4192"},"modified":"2016-05-26T20:07:38","modified_gmt":"2016-05-26T20:07:38","slug":"lab-protocols","status":"publish","type":"page","link":"http:\/\/blogs.rochester.edu\/larracuente\/lab-protocols\/","title":{"rendered":"Lab protocols"},"content":{"rendered":"<p>Below are links to commonly used Larracuente Lab protocols.<\/p>\n<figure id=\"attachment_4322\" aria-describedby=\"caption-attachment-4322\" style=\"width: 173px\" class=\"wp-caption alignright\"><a href=\"https:\/\/blogs.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/2_slot32sec.jpeg\" rel=\"attachment wp-att-4322\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4322\" src=\"https:\/\/blogs.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/2_slot32sec-300x208.jpeg\" alt=\"2_slot32sec\" width=\"173\" height=\"120\" srcset=\"https:\/\/blogs.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/2_slot32sec-300x208.jpeg 300w, https:\/\/blogs.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/2_slot32sec.jpeg 360w\" sizes=\"auto, (max-width: 173px) 100vw, 173px\" \/><\/a><figcaption id=\"caption-attachment-4322\" class=\"wp-caption-text\">Slot blot showing copy number variation of\u00a0 repeats in Drosophila melanogaster. Credit: D. Eickbush<\/figcaption><\/figure>\n<p><a href=\"https:\/\/blogs.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/LarracuenteLab.Squish.Preps_.pdf\" rel=\"\">Squish prep <\/a><\/p>\n<p><a href=\"https:\/\/blogs.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/LarracuenteLab.Squish_Plus_Preps.pdf\" rel=\"\">&#8220;Squish +&#8221; prep<\/a><\/p>\n<p><a href=\"https:\/\/blogs.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/LarracuenteLab.Slot_blotting_DNA.pdf\" rel=\"\">DNA slot blots<\/a><\/p>\n<p><a href=\"https:\/\/blogs.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/LarracuenteLab.RNA_Isolation.pdf\" rel=\"\">Total RNA isolation<\/a><\/p>\n<p><a href=\"https:\/\/blogs.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/LarracuenteLab.Chemiluminscence.pdf\" rel=\"\">Chemiluminescent hybridization and <\/a><a href=\"https:\/\/blogs.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/LarracuenteLab.Chemiluminscence.pdf\" rel=\"\">detection<\/a><\/p>\n<figure id=\"attachment_796\" aria-describedby=\"caption-attachment-796\" style=\"width: 146px\" class=\"wp-caption alignright\"><a href=\"https:\/\/blogs.rochester.edu\/larracuente\/wp-content\/uploads\/2014\/12\/359_jpeg.jpg\" rel=\"attachment wp-att-796\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-796\" src=\"https:\/\/blogs.rochester.edu\/larracuente\/wp-content\/uploads\/2014\/12\/359_jpeg.jpg\" alt=\"359_jpeg\" width=\"146\" height=\"146\" srcset=\"https:\/\/blogs.rochester.edu\/larracuente\/wp-content\/uploads\/2014\/12\/359_jpeg.jpg 168w, https:\/\/blogs.rochester.edu\/larracuente\/wp-content\/uploads\/2014\/12\/359_jpeg-150x150.jpg 150w\" sizes=\"auto, (max-width: 146px) 100vw, 146px\" \/><\/a><figcaption id=\"caption-attachment-796\" class=\"wp-caption-text\">FISH showing the hybridization of a 1.688 satellite DNA probe on D. melanogaster mitotic chromosomes.<\/figcaption><\/figure>\n<p><a href=\"https:\/\/blogs.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/Larracuente_Ferree_2015.pdf\" rel=\"\">FISH on squashed tissue<\/a><\/p>\n<p>&nbsp;<\/p>\n<h3><strong>Computational protocols<\/strong><\/h3>\n<p>Please visit our GitHub page for computational pipelines: <a href=\"https:\/\/github.com\/LarracuenteLab\/\" target=\"_blank\">https:\/\/github.com\/LarracuenteLab\/<\/a>.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Below are links to commonly used Larracuente Lab protocols. Squish prep &#8220;Squish +&#8221; prep DNA slot blots Total RNA isolation Chemiluminescent hybridization and detection FISH on squashed tissue &nbsp; Computational protocols Please visit our GitHub page for computational pipelines: https:\/\/github.com\/LarracuenteLab\/. &nbsp; &nbsp;<\/p>\n","protected":false},"author":26,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-4192","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/blogs.rochester.edu\/larracuente\/wp-json\/wp\/v2\/pages\/4192","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.rochester.edu\/larracuente\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/blogs.rochester.edu\/larracuente\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.rochester.edu\/larracuente\/wp-json\/wp\/v2\/users\/26"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.rochester.edu\/larracuente\/wp-json\/wp\/v2\/comments?post=4192"}],"version-history":[{"count":14,"href":"https:\/\/blogs.rochester.edu\/larracuente\/wp-json\/wp\/v2\/pages\/4192\/revisions"}],"predecessor-version":[{"id":5492,"href":"https:\/\/blogs.rochester.edu\/larracuente\/wp-json\/wp\/v2\/pages\/4192\/revisions\/5492"}],"wp:attachment":[{"href":"https:\/\/blogs.rochester.edu\/larracuente\/wp-json\/wp\/v2\/media?parent=4192"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}