Following is the paper we are going to talk about on Friday.

Differences in forward angular light scattering distributions between M1 and M2 macrophages

And if you have some notes or comments, you could comment in our blog, under this post!

Following is the paper we are going to talk about on Friday.

Differences in forward angular light scattering distributions between M1 and M2 macrophages

And if you have some notes or comments, you could comment in our blog, under this post!

These days work are mainly about comparing the performance of normal fitting and direct fitting.

A review for whole process. We have a parent Gauss or Normal distribution(parameter μ and σ). In math that should be continuous. Then we sample the distribution got a set of discrete data. Then we use the discrete population to map to a set of scattergram that generate by Mie scatter thoery in different particle diameter. After that we use cross-section to weight the data. Now we get the raw simulation data we use in normal fitting.

In normal fitting we use log-normal population model + cross-section weighted model+Mie theory to fit to the scattergram. We want to report best estimate of I vs d.

For direct fitting, the raw simulation data generate is similar to the former one. We have a parent Gauss or Normal distribution(parameter μ and σ), then we sample the distribution get a set of discrete data. We weighted these data though cross-weighting model. Now we get the raw simulation data.

In direct fitting, we use a log-normal or gaussian population model to fit raw data directly(without Mie thoery, that is why we call it direct fit).

The purpose of normal fitting is we want to analyze cell’s angular scattergram we taking from our experiment devices. We would like to report best estimate of I vs d.

The purpose of comparing these two fitting process is we want to know several things: how well our normal fit compared to the direct fit; when the samples are very sparse, the Mie thoery model’s contribution could be neglected, and so on.

Followings are several simulation results for comparing direct fitting and normal fitting. Continue reading Compare normal fitting with direct fitting